Pair Name	Vanillin, Fluorouracil
Disease Info	[ICD-11: 2B91.Z]	Colorectal cancer		Investigative
Biological Phenomena	Induction-->Apoptosis
Gene Regulation	Down-regulation	Expression	NNMT	hsa4837
	Up-regulation	Expression	TP53	hsa7157
	Up-regulation	Cleavage	CASP3	hsa836
	Up-regulation	Cleavage	CASP9	hsa842
	Up-regulation	Cleavage	PARP1	hsa142
	Up-regulation	Phosphorylation	MAPK14	hsa1432
	Up-regulation	Phosphorylation	MAP3K5	hsa4217
In Vitro Model	HT-29	Colon adenocarcinoma	Homo sapiens (Human)	CVCL_0320
	SW480	Colon adenocarcinoma	Homo sapiens (Human)	CVCL_0546
In Vivo Model	A total of 3×10⁶ cells from each of the SW480/NC and SW480/NNMT cell lines were subcutaneously injected into nude mice (n=5 per cell line).
Result	Vanillin is deemed to be a promising anticancer candidate by inhibiting NNMT and may attenuate NNMT‑induced resistance to 5‑Fu in human CRC therapy with few side effects.